This study investigated production of biodiesel from oil seeds of Jatropha curcas, obtained in Nigeria, with a view to encouraging the cultivation of Jatropha plant in Nigeria and to providing a motivation for the development of biodiesel production from this feedstock. Also effect of oil-to-alcohol molar ratio on the biodiesel production from Nigerian Jatropha curcas seeds oil was investigated with a view to confirming established base case. A maximum yield of fatty acid methyl esters (FAME) of 87% was obtained at 333 Kelvin, oil-to-alcohol molar ratio of 1:6 and at 1wt% NaOH catalyst concentration. The physical properties of the BD obtained from Nigerian Jatropha oil met the ASTM standard of D-6751. Consequently, this study reveals that Jatropha curcas, an economically invaluable plant in Nigeria, could be a good feedstock for biodiesel production in Nigeria.