Objectives: The aims of this study were to perform the 20-meter shuttle run test (Bleep test) and the Yo-Yo test in two high level British female basketball teams, and to evaluate and compare both tests between themselves. Methods: 14 elite level female basketball players from a top-4 team competing in the England Basketball League Division I and 15 female basketball players from the Under 20 Great Britain National Team (U20 GB) playing in the European Championship voluntarily participated in this study. The anthropometry tests performed were weight, height and BMI. Each team performed the Bleep test and the Yo-Yo Test on two separate and non-consecutive days (with a lapse of 48 hours between the two tests), at the same time (10 am) and in the same gym hall with wooden floor. Results: Statistical differences were found between the VO2max values of the two teams obtained in the 20-meter shuttle run (p=0.000), and between the VO2max calculated by Yo-Yo IR1 and the VO2max calculated by 20-meter shuttle run without taking into account the age (p=0.002). Conclusions: British basketball players showed cardiorespiratory levels (VO2max) lower than high-level female basketball players from countries where basketball is more popular and better developed. In addition, it was proved that there are no differences when it comes to the use of the Bleep test or the Yo-Yo test in order to calculate the maximum oxygen uptake through an indirect method.