This study focuses and discusses the effects of temperature and humidity on electrospun conductive nanofibers, made with different polymer blends, deposited directly on interdigitated electrodes. The selected conductive polymers were based on blends of polyaniline emeraldine salt form and three different carrier hosting polymers: polyvinilpyrrolidone, polystyrene, and polyethylene oxide respectively. The obtained fibrous layers were investigated by the electrical measurements and morphological analysis (scanning electron microscopy). The study was made on the correlation between the electrical changes and morphological discrepancies due to temperature treatment. Moreover, this article reports the effects of relative humidity variations on electrical parameters. Since polyaniline is a well-known sensing material for different gases and volatile organic compounds, this study could be considered a supportive study for employing of the mentioned polymer blends as chemical interactive materials in gas sensor applications.