We have successfully demonstrated large-area and continuous MoS2 films grown on indium tin oxide (ITO) substrates by RF sputtering followed by a post-annealing process. Thin Cu layers were prepared on MoS2/ITO films by thermal evaporation to enhance electron-transfer rates as well as the catalytic activity of hydrazine oxidation. High electrocatalytic activity towards hydrazine oxidation was observed in a Cu/MoS2/ITO hybrid structure. A large residual current was also observed in the Cu/MoS2/ITO hybrid due to the combination of MoS2 and Cu film. The electrocatalytic activity was enhanced with the increase of the MoS2 film thickness. The observed results showed that the Cu/MoS2/ITO catalyst is indeed a valuable material for future applications in hydrazine fuel cells.