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Optica, Optics Letters, 21(38), p. 4510, 2013

DOI: 10.1364/ol.38.004510

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Resolution and contrast enhancement in coherent anti-Stokes Raman-scattering microscopy

Journal article published in 2013 by Alicja Gasecka, Amy Daradich, Harold Dehez, Michel Piché, Daniel Côté ORCID
This paper is available in a repository.
This paper is available in a repository.

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Abstract

We implement switching laser mode coherent anti-Stokes Raman-scattering (SLAM-CARS) microscopy to enhance the spatial resolution and contrast in label-free vibrational microscopy. The method, based on the intensity difference between two images obtained with Gaussian and doughnut-shaped modes, does not depend on the specimen and relies on minimal modifications of the typical CARS setup. We demonstrate subdiffraction resolution imaging of myelin sheaths in a mouse brainstem. A lateral resolution of 0.36λp is achieved.