The essential oil from the aerial parts of Artemisia rutifolia Stephan ex Spreng., collected from two different regions of Tajikistan, were obtained by hydrodistillation and analyzed by GC-MS. A total of 77 compounds were identified in the oils, accounting for 98.6 % and 99.2 % of the two oils. Both essential oils were dominated by α-thujone (20.9 % and 36.6 %) and β-thujone (47.3 % and 36.1 %) with lesser amounts of 1,8-cineole (3.2 % and 11.7 %) and germacrene D (2.8 % and 1.8 %).