We describe a new species of whiptail lizard genus Ameivula from Planalto dos Gerais, a sandstone plateau that extends along the Cerrado region in the states of Bahia, Tocantins, Piauí, and Minas Gerais, in Brazil. The new species is the third recognized species of the A. ocellifera group registered for the Cerrado. Quantitative analyses of morphometric characters showed that Caatinga species are distinguished from their Cerrado congeners on basis of body size and shape. A discriminant analysis upon meristic characters showed that the new species can be clearly distinguished from the other two Cerrado species A. mumbuca and A. jalapensis, also the morphology of the finger lamellae, and clutch size distinguish these species.