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American Chemical Society, Analytical Chemistry, 16(85), p. 7889-7896, 2013

DOI: 10.1021/ac4015325

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In Vivo Fluorescent Adenosine 5'-Triphosphate (ATP) Imaging of Drosophila melanogaster and Caenorhabditis elegans by Using a Genetically Encoded Fluorescent ATP Biosensor Optimized for Low Temperatures

Journal article published in 2013 by Taiichi Tsuyama, Han Yw, Jun-Ichi Kishikawa ORCID, Yong-Woon Han, Yoshie Harada, Asako Tsubouchi ORCID, Hiroyuki Noji, Akira Kakizuka, Ken Yokoyama ORCID, Tadashi Uemura, Hiromi Imamura
This paper is available in a repository.
This paper is available in a repository.

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Abstract

Adenosine 5'-triphosphate (ATP) is the major energy currency of all living organisms. Despite its important functions, the spatio-temporal dynamics of ATP levels inside living multi-cellular organisms is unclear. In this study, we modified the genetically encoded Förster resonance energy transfer (FRET)-based ATP biosensor ATeam to optimize its affinity at low temperatures. This new biosensor, AT1.03NL, detected ATP changes inside Drosophila S2 cells more sensitively than the original biosensor did, at 25°C. By expressing AT1.03NL in Drosophila melanogaster and Caenorhabditis elegans, we succeeded in imaging the In vivo ATP dynamics of these model animals at single-cell resolution.