The aim of this study was to establish the validity of the anaerobic threshold (AT) determined on the soccer specific Hoff circuit (ATHoff) in order to predict the maximal lactate steady state exercise intensity (MLSSHoff) with the ball. Sixteen soccer players (16.0±0.5 years; 63.7±9.0 kg, and 169.4±5.3 cm) were submitted to five progressive efforts (7.0 to 11.0 km·h), with ball dribbling. Thereafter, eleven players were submitted to three efforts of 30min at 100, 105, and 110% of ATHoff. The ATHoff corresponded to the speed relative to 3.5 mmol L lactate concentration. The speed relative to 4.0 mmol.L was assumed to be ATHoff4.0 and the ATHoffBI was determined through bi-segmented adjustment. For comparisons, Student t-test, intraclass correlation, and Bland & Altman analyzes were used. For reproducibility, intraclass correlation, typical error, and coefficient of variation were used. No significant difference was found between AT test and retest determined using different methods. A positive correlation was observed betweenATHoffand ATHoff4.0. The MLSSHoff (10.6±1.3 km h) was significantly different compared to ATHoff (10.2±1.2 km h) and ATHoffBI (9.5±0.4 km h) but did not show anydifference from LAnHoff4.0 (10.7±1.4 km.h). The MLSSHoff presented high intraclass correlations coefficients with ATHoffand ATHoff4.0 (ICC = 0.94; and ICC=0.89; p < 0.05, respectively), without significant correlation with ATHoffBI. The results suggest that AT determined on the Hoff circuit is reproducible and capable of predicting MLSS. The ATHoff4.0 was the method which presented a better approximation to MLSS. Therefore, it is possible to assess submaximal physiological variables through a specific circuit performed with the ball in young soccer players.