A study was conducted to examine the effect of pH on the uptake of NH4 + and NO3 - by the cell suspension culture of Vigna radiata. Uptake of NH4 + was faster than NO3 - under all conditions of the medium whereas uptake of NH4 + and NO3 - both were affected by the pH of the medium. Maximum uptake of NH4 + was observed on pH 4.5. Uptake of NO3 - was faster in the culture having pH 7.0. It is therefore concluded that pH of the medium influence the uptake of both NH4 + and NO3 - in the cell suspension culture of Vigna radiata.