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Sociedade Brasileira de Microbiologia, Brazilian Journal of Microbiology, 2(43), p. 602-605

DOI: 10.1590/s1517-83822012000200023

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Diagnosis of canine leptospirosis using an immunomagnetic separation-PCR method

This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Diagnosis of leptospirosis by PCR is hampered due to the presence of substances on biological fluids. Here, we report an immunomagnetic separation step prior to PCR which improved the detection of Leptospira spp. in blood and urine samples from dogs. It resulted in a significant improvement on sensitivity for diagnosis of canine leptospirosis.