An enzyme free potentiometric glucose sensor was developed through the electrochemical preparation of a new poly (3-aminophenyl boronic acid-co-3-octylthiophene) (PAPBAOT) organic electrode. A PAPBAOT conducting polymer film electrode was first synthesized electrochemically on glassy carbon electrode and the analytical performance of the developed sensor was evaluated using potentiometric measurements. Boronic acid and the alkyl spacer functional group were used as molecular recognition and penetration agent, respectively. The PAPBAOT film electrode was characterized using cyclic voltammetry, Scanning electron microscopy (SEM), UV–vis spectroscopy, Raman spectroscopy, and Differential scanning calorimetry (DSC). The potentiometric calibration plot showed a hydrodynamic range of 5–50 mM glucose within a range of blood glucose level. The detection limit was 0.5 mM, and the sensor exhibited no interference from common interference such as ascorbic acid, dopamine, and uric acid. The PAPBAOT film electrode was used for the determination of glucose in human plasma samples and the results were compared with commercial amperometric sensors.