In this work, we have demonstrated that the solvent plays a critical role in the nanotubes formation process which can be controlled by the ambient temperature. It is believed that the ambient temperature and the rate of evaporation of the solvent during the electrospinning process are the major factors controlling the formation of different electrospun nanofibre morphologies. By their very nature, electrospun different nanofibre morphologies depend on the competition between the phase separation dynamics and the evaporation rate of solvent controlled by the phase diagram of the polymer solution. In our experiments, MgFe2O4 nanotubes are prepared by using different solvent mixtures at a specific ambient temperature (ethanol at room temperature and deionized water homogeneous temperature field of 45 degrees C) by single capillary electrospinning process, and compared with MgFe2O4 nanofibers obtained using deionized water at room temperature. A possible formation mechanism, morphology template effect combined with phase separation theory, is proposed to interpret the formation process of the MgFe2O4 nanotubes. Detailed morphology, structural characterizations show that individual MgFe2O4 nanotubes are made of MgFe2O4 nanocrystals stacking along the nanotubes with no preferred growth directions and individual nanocrystals are single crystal with a cubic spinel structure. In addition, the magnetic properties of MgFe2O4 nanotubes calcined at different temperature will be reported and discussed.