In this communication, a laminated, flexible, microfluidic-integrated, all CNT based liquid-gated transistor and biosensor are reported that comprises single walled CNTs for both the semiconducting channel as well as the contact electrodes. The proposed architecture eliminates the need for lithography, electrode definition processes, and also circumvents substrate surface compatibility issues. Real-time detection of 1 pM poly-L-lysine in a liquid-gated transistor comprising only two materials, single walled CNTs and polydimethoxysilane substrate with microfluidic channel, is demonstrated.