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Wiley, International Journal of Cancer, 5(111), p. 720-726, 2004

DOI: 10.1002/ijc.20310

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Non-self-discrimination as a driving concept in the identification of an immunodominant HMW-MAA epitopic peptide sequence by autoantibodies from melanoma cancer patients

Journal article published in 2004 by Reinhard Dummer, Abraham Mittelman, Francesco P. Fanizzi ORCID, Fanizzi Fp, Guglielmo Lucchese, Jörg Willers, Darja Kanduc
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

We analyzed the sera of patients with melanoma to define the human humoral autoantibody profile towards HMW-MAA. Computational proteome scanning using the non-self-discrimination principle as a guide led to the individuation of the low-similarity HMW-MAA781-789RATVWMLRL peptide fragment as an immunodominant B-cell epitope. Linear B-cell determinant individuation was experimentally validated by dot blot immunoassay and NMR spectroscopy analysis. Regulation of physiologic self-reactivity by the non-self-discrimination principle is discussed.