Dissemin is shutting down on January 1st, 2025

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Bull. Fr. Pêche Piscic., 385, p. 17-24

DOI: 10.1051/kmae:2007002

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Lack of specificity of the molecular diagnostic method for identification of Aphanomyces astaci

Journal article published in 2007 by I. Ballesteros, M. P. Martín ORCID, L. Cerenius, K. Söderhäll, M. T. Tellería, Javier Diéguez-Uribeondo
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

A recent PCR-test developed for identification of Aphanomyces astaci, the organism responsible for crayfish plague, provided false positives for Aphanomyces frigidophilus, Aphanomyces repetans, and some Saprolegnia spp. Real-time PCR showed that with the designed primers, A. astaci and A. frigidophilus cannot be distinguished. The results of this study show that this particular crayfish plague PCR-test ought to be improved and that molecular-based techniques need to be contrasted to histological evidences and disease history.