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Wiley, ChemBioChem, 18(9), p. 2958-2961, 2008

DOI: 10.1002/cbic.200800604

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Segmental Isotopic Labelling of a Multidomain Protein by Protein Ligation by Protein Trans-Splicing

Journal article published in 2008 by Mikko Muona ORCID, A. Sesilja Aranko, A. Sesilja Aranko, Hideo Iwai
This paper is available in a repository.
This paper is available in a repository.

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Abstract

Structure–function relationships of intact multidomain proteins: Segmental isotopic labelling of multidomain proteins could open a new avenue for studying particular domains in intact proteins, because it could simplify the NMR spectra through the incorporation of stable isotopes in a region-specific manner. We have demonstrated that the necessary protein ligation can be easily achieved both in vivo and in vitro by protein trans-splicing.