Published in

Wiley, ChemBioChem, 18(9), p. 2958-2961

DOI: 10.1002/cbic.200800604

Links

Tools

Export citation

Search in Google Scholar

Segmental Isotopic Labelling of a Multidomain Protein by Protein Ligation by Protein Trans-Splicing

Journal article published in 2008 by Mikko Muona ORCID, A. Sesilja Aranko, A. Sesilja Aranko, Hideo Iwai
This paper is available in a repository.
This paper is available in a repository.

Full text: Download

Green circle
Preprint: archiving allowed
Orange circle
Postprint: archiving restricted
Red circle
Published version: archiving forbidden
Data provided by SHERPA/RoMEO

Abstract

Structure–function relationships of intact multidomain proteins: Segmental isotopic labelling of multidomain proteins could open a new avenue for studying particular domains in intact proteins, because it could simplify the NMR spectra through the incorporation of stable isotopes in a region-specific manner. We have demonstrated that the necessary protein ligation can be easily achieved both in vivo and in vitro by protein trans-splicing.