The European grapevine moth Lobesia botrana Denis & Schiffermüller (Lepidoptera: Tortricidae) is a major pest on grapes worldwide. Attempts to develop control methods for this pest based on grape kairomones demonstrate limited success and studies indicate that a major limiting factor is overlap between synthetic kairomones and background odours in the vineyard. Behaviourally active compounds from non-host plants may thus represent an effective alternative for monitoring and control methods. Extracts from food plants (i.e. from capsicum, garlic and peppermint, which elicit the so-called somatosensory sensation) are traditionally used in agriculture for the control of pest insects. Among those plants, Perilla frutescens L. (Lamiales: Lamiaceae), native of Asia, contains compounds activating sensory ion channels in mammals, which are known to be involved in the perception of somatosensory compounds and are expressed in tortricid moth antennae. In the present study, in search of non-host volatiles with potential application in pest control, essential oil metabolites isolated from P. frutescens are screened for biological activity on the olfactory system of L. botrana. The compounds (S)-(−)-perillaldehyde and isoegomaketone, which are released from different P. frutescens varieties, are identified by gas chromatography-coupled electroantennographic detection. In a dual-choice oviposition test, females show a preference for a combination of host odours and perillaldehyde, preferring this over a host-plant odour bouquet alone. In Y-tube olfactometer assays, virgin males show a higher level of activity in the presence of isoegomaketone, even if not significantly responsive to the compound.