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Springer Nature [academic journals on nature.com], Oncogene, 41(21), p. 6387-6394, 2002

DOI: 10.1038/sj.onc.1205751

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Isolation of HELAD1, a novel human helicase gene up-regulated in colorectal carcinomas

Journal article published in 2002 by Hideyuki Ishiguro, Takashi Shimokawa, Tatsuhiko Tsunoda, Toshihiro Tanaka ORCID, Yoshitaka Fujii, Yusuke Nakamura, Yoichi Furukawa
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

To investigate the mechanisms of colorectal carcinogenesis, we searched for genes regulated by adenomatous polyposis coli gene product (APC) and identified a novel gene, termed HELAD1 (helicase, APC down-regulated 1). A recombinant polypeptide representing the ATPases associated with cellular activities (AAA) domain of the HELAD1 product showed 3' to 5' helicase activity and exonuclease activity in vitro. HELAD1 was abundantly expressed in 16 of 20 colon cancers examined but hardly detectable in corresponding non-cancerous mucosae. Treatment of colon-cancer cells with antisense oligonucleotides suppressed its expression and induced apoptosis. These data revealed an importance of HELAD1 in colorectal carcinogenesis and suggest that suppression of HELAD1 may be a promising therapeutic strategy.