About ten years ago, NIR technology was implemented at the Colombian Sugarcane Research Center Cenicaña for the analysis of sugarcane quality, followed by the development of methodologies for the quantification of some elements in soils and sugarcane leaves. Methodologies were based on the presence of high content materials, such nitrogen, potassium, calcium, and magnesium; however, determination of micronutrients which are found in soils and leaves in the range of parts per million has been challenging. Development of a NIR methodology for quantification of macro (N, P, K, Ca, Mg) and micronutrients (Cu, Zn, Mn, Fe) in a single experiment is here reported. Calibration curves were constructed using approximately 500 samples that were previously analyzed by methods of reference such atomic absorption. Statistical analysis of the data showed that there are not significant differences between the methods of reference and NIR, suggesting that NIR is a very fast, economical and convenient methodology for the daily analysis of sugarcane leaves.