1-Butanol (or bio-butanol) was recently proposed to be used with gasoline, and engine tests were performed. However, only few studies concern the oxidation/combustion of 1-butanol and they do not provide directly usable data for kinetic modeling. Thus the oxidation of butanol–gasoline surrogate mixtures (85–15 vol.%) was studied using a jet-stirred reactor. One representative of the chemical classes constituting this fuel was selected. These constituents were iso-octane, toluene, 1-hexene and 1-butanol. The experiments were carried out in the temperature range 770–1220 K, at 10 atm, with an initial fuel concentration of 0.1% in mole. Four equivalence ratios were considered: 0.3, 0.6, 1, and 2. Concentration profiles of reactants, stable intermediates, and final products were measured via sonic probe sampling followed by FTIR and gas chromatography analyses. A detailed chemical kinetic scheme (251 species, 1990 reversible reactions) resulting from the merging of validated kinetic schemes for the oxidation of the pure components of the present butanol–gasoline surrogate was used to simulate the present experiments. Good agreement between the experimental results and the computations was observed under the present JSR conditions.