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Wiley, Angewandte Chemie International Edition, 18(51), p. 4431-4434, 2012

DOI: 10.1002/anie.201200923

Wiley, Angewandte Chemie, 18(124), p. 4507-4510, 2012

DOI: 10.1002/ange.201200923

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Triple Bioorthogonal Ligation Strategy for Simultaneous Labeling of Multiple Enzymatic Activities

Journal article published in 2012 by Lianne I. Willems, L. I.; Li, Gijsbert A. van der Marel, Nan Li, N.; Florea, Bogdan I. Florea, B. I.; Ruben, Mark Ruben, M.; Van der Marel, G. A.; Overkleeft, H. S., Herman S. Overkleeft ORCID
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This paper is available in a repository.

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Abstract

Three at the same time: A ligation strategy combining tetrazine-norbornene cycloaddition, Staudinger-Bertozzi ligation, and copper(I)-catalyzed click reaction was used to label the three catalytic activities of the proteasome simultaneously in a single experiment. The orthogonality of the three ligation reactions enables selective monitoring of multiple targets at the same time in complex biological samples.