Wiley, Angewandte Chemie International Edition, 18(51), p. 4431-4434, 2012
Wiley, Angewandte Chemie, 18(124), p. 4507-4510, 2012
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Three at the same time: A ligation strategy combining tetrazine-norbornene cycloaddition, Staudinger-Bertozzi ligation, and copper(I)-catalyzed click reaction was used to label the three catalytic activities of the proteasome simultaneously in a single experiment. The orthogonality of the three ligation reactions enables selective monitoring of multiple targets at the same time in complex biological samples.