Hypertension (HTA) is an important component of the metabolic syndrome. The prevalence of this syndrome is highly increased by HTA. The first step for MS treatment is a change of life-style, being the diet one of the main factors. It is known that a diet based on vegetables and fruits, improves cardiovascular diseases and MS, which is attributed to its polyphenol content. Specially, our research group has demonstrated that procyanidins, which is a class of polyphenols, form grape seed improve lipid metabolism, insulin resistance, oxidative stress and inflammatory state in MS. Therefore, the aim of this study was to evaluate the antihypertensive effect of a grape seed procyanidin extract (GSPE) in cafeteria diet-induced hypertensive rats. Moreover, the contribution of nitric oxide (NO), an endothelium-derived vasodilator factor, was also studied. Male Wistar cafeteria-fed rats (n=10 per group) were administrated by intragastric intubation GSPE (250, 375 and 500mg/Kg of body weight) and systolic blood pressure (SBP) was recorded by the tail-cuff method at 0, 2, 4, 6, 8, 24, and 48h post-administration. Water and Captopril (50 mg/Kg bw), a known antihypertensive drug, were used as controls. To determine NO contribution, the animals (n=7 per group), were administered GSPE (375mg/kg bw) by intragastric gavage. 4h post-administration, L-NAME (30mg/kg bw) or saline were injected intraperitoneally and SBP were recorded at 0h and 6h post-administration. GSPE produced a dose-dependent decrease in SBP up to the dose of 375 mg/Kg bw. The maximum decrease was achieved 6h post-administration, which was similar to the reached with Captopril. Interestingly, using higher doses of GSPE the decrease in SBP was not so effective. Furthermore, the antihypertensive effect of GSPE was completely abolished with L-NAME.