Genetic diversity is positively associated with plant fitness, stability, and the provision of ecosystem services. Preserving genetic diversity is therefore considered an important component of ecosystem restoration as well as a measure of its success. We examined the genetic diversity of restored Zostera marina meadows in a coastal bay system along the USA mid-Atlantic coast using microsatellite markers to compare donor and recipient meadows. We show that donor meadows in Chesapeake Bay have high genetic diversity and that this diversity is maintained in meadows restored with seeds in the Virginia coastal bays. No evidence of inbreeding depression was detected (FIS −0.2 to 0) in either donor or recipient meadows, which is surprising because high levels of inbreeding were expected following the population contractions that occurred in Chesapeake Bay populations due to disease and heat stress. Additionally, there was no evidence for selection of genotypes at the restoration sites, suggesting that as long as donor sites are chosen carefully, issues that diminish fitness and survival such as heterosis or out-breeding depression can be avoided. A cluster analysis showed that, in addition to the Chesapeake Bay populations that acted as donors, the Virginia coastal bay populations shared a genetic signal with Chincoteague Bay populations, their closest neighbor to the north, suggesting that natural recruitment into the area may be occurring and augmenting restored populations. We hypothesize that the high genetic diversity in seagrasses restored using seeds rather than adult plants confers a greater level of ecosystem resilience to the restored meadows. ; Laura K. Reynolds, Michelle Waycott, Karen J. McGlathery, Robert J. Orth, Joseph C. Zieman