Elsevier, Sensors and Actuators B: Chemical, (195), p. 246-251, 2014
DOI: 10.1016/j.snb.2014.01.026
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A novel thiol-reactive fluorescence probe based on the 1,8-naphthalic anhydride fluorophore was designed and synthesized that selectively detected thiols over other relevant biological species by the turning on of its fluorescence through a Michael addition reaction. In aqueous buffer solutions at physiological pH, the probe exhibited 4-fold enhancement in fluorescence intensity by a Michael addition of Cys, Hey, and GSH to the maleimide appended to a naphthalic. Cell permeability and live cell imaging of thiols are also demonstrated.