Dissemin is shutting down on January 1st, 2025

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Wiley, Parasite Immunology, 5(36), p. 199-206, 2014

DOI: 10.1111/pim.12104

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Signatures of malaria vaccine efficacy in ageing murine immune memory

This paper is available in a repository.
This paper is available in a repository.

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Data provided by SHERPA/RoMEO

Abstract

Malaria transmission occurs by mosquito bite. Thereafter, Plasmodium sporozoites specifically invade the liver, where they develop into thousands of merozoites that initiate blood stage infection and clinical malaria. The pre-erythrocytic phase of a Plasmodium infection is the target of experimental whole parasite vaccines against malaria. Repeated immunizations with high doses of live, metabolically active sporozoites can induce protracted protection against Plasmodium reinfection. Parasites lacking a Plasmodium-specific apicoplast protein, termed PALM, arrest very late during intra-hepatic development just prior to liver merozoite release and can elicit sterile protection with two immunization doses only. In this report, we show in the robust Plasmodium berghei-C57BL/6 model that partial protection extends beyond one year after the last immunization. In aging mice, intracellular cytokine staining of Plasmodium peptide-stimulated intra-hepatic CD8(+) T cells revealed elevated levels of interferon gamma in vaccinated mice. We conclude that antigen-specific T cells persist in the target organ and are critical signatures of lasting protection. Our data also supports the notions that memory T cell responses generated early in life remain largely intact well into old age and that murine Plasmodium vaccination and infection models are suitable to study the mechanisms of maintenance and efficiency of adaptive immunity during immunosenescence. This article is protected by copyright. All rights reserved.