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Ivyspring International Publisher, Theranostics, 8(5), p. 796-804

DOI: 10.7150/thno.11220

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On Chip Analysis of CNS Lymphoma in Cerebrospinal Fluid

Journal article published in 2015 by Anna Turetsky, Kyungheon Lee, Jun Song, Randy J. Giedt, Eunha Kim, Alexandra E. Kovach ORCID, Ephraim P. Hochberg, Cesar M. Castro, Hakho Lee, Ralph Weissleder ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Molecular profiling of central nervous system lymphomas in cerebrospinal fluid (CSF) samples can be challenging due to the paucicellular and limited nature of the samples. Presented herein is a microfluidic platform for complete CSF lymphoid cell analysis, including single cell capture in sub-nanoliter traps, and molecular and chemotherapeutic response profiling via on-chip imaging, all in less than one hour. The system can detect scant lymphoma cells and quantitate their kap- pa/lambda immunoglobulin light chain restriction patterns. The approach can be further custom- ized for measurement of additional biomarkers, such as those for differential diagnosis of lym- phoma subtypes or for prognosis, as well as for imaging exposure to experimental drugs.