Ivyspring International Publisher, Theranostics, 8(5), p. 796-804
DOI: 10.7150/thno.11220
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Molecular profiling of central nervous system lymphomas in cerebrospinal fluid (CSF) samples can be challenging due to the paucicellular and limited nature of the samples. Presented herein is a microfluidic platform for complete CSF lymphoid cell analysis, including single cell capture in sub-nanoliter traps, and molecular and chemotherapeutic response profiling via on-chip imaging, all in less than one hour. The system can detect scant lymphoma cells and quantitate their kap- pa/lambda immunoglobulin light chain restriction patterns. The approach can be further custom- ized for measurement of additional biomarkers, such as those for differential diagnosis of lym- phoma subtypes or for prognosis, as well as for imaging exposure to experimental drugs.