Nitric oxide (NO) is an important component of biogeochemical cycling of nitrogen, produced via biologically mediated processes of nitrification and denitrification in soils. The production and consumption processes of NO in black soils are not fully understood. We established how moisture and temperature affect NO dynamics for black soil samples of maize land in the temperate zone of northeastern China. The optimum soil moisture for the maximum NO production and emission was determined to be 41% water-filled pore space (WFPS), based on laboratory experiments and modeling. For a given moisture, NO fluxes increased exponentially with soil temperature at any given soil moisture. The optimum soil moisture for the maximum NO emission was constant and independent of soil temperature. The NO consumption rate constant (k) in the studied soil (range 9.31 × 10−6–15.1 × 10−6 m3 kg−1 s−1) was in the middle of the range of similar k values published to date. The maximum NO emission potential for black soils at 25 °C and 15 °C were about 18.6 and 9.0 ng N m−2 s−1, respectively. Based on laboratory results and field monitoring data of soil water content and soil temperature, the average NO fluxes from black soils in the region were estimated to be 10.7 ng N m−2 s−1 for an entire plant growth period. NO emissions likely occur principally in July, associated with optimum soil moisture. The present study suggests that NO fluxes from black soil are much lower than the previous reports from cropland in southern parts of China.