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Springer (part of Springer Nature), Analytical and Bioanalytical Chemistry, 1(390), p. 61-65

DOI: 10.1007/s00216-007-1432-7

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Isotopic labelling of peptides and isotope ratio analysis using LC-ICP-MS: A preliminary study

This paper is available in a repository.
This paper is available in a repository.

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Abstract

Bradykinin and substance P have been derivatised with cyclic diethylenetriaminepentaacetic anhydride (cDTPA) and subsequently labelled with natural and isotopically enriched Eu(3+). This enabled the detection and relative quantitation of the peptides using element-selective detection by high-performance liquid chromatography inductively coupled plasma mass spectrometry (LC-ICP-MS). Relative quantitation was achieved by differentially labelling two peptide sources, after derivatisation with cDTPA, using natural and enriched (151)Eu respectively. The (151)Eu:(153)Eu isotope ratio was measured and used to calculate the original peptide ratio. The measured ratios came within 5.2% of the known ratio. Derivatisation and chelation reactions were additionally confirmed using LC-ESI-MS.