A sensitive electrochemical active interface for sugar sensing based on the specific boronic acid-diol binding was established. The sensing matrix was formed by stirring a suspension of graphene oxide (GO) with 4-aminophenylboronic acid (APBA). The resulting composite consists of a water insoluble precipitate of reduced graphene oxide (rGO) with APBA incorporated into the rGO matrix. Differential pulse voltammetry (DPV) on glassy carbon electrodes modified with rGO/APBA was used for the detection of fructose, mannose and glucose. The fabricated sensor exhibited a wide linear range with detection limits of 100nM for fructose, and around 800nM for mannose and glucose.