Glycan expression pattern in the tissue of marine organisms can provide useful information about glycobiology from different angles. The diversity of oligosaccharide structures present in the annelid from the Pacific Ocean, Perinereis nuntia ver. vallata (polychaeta), was surveyed by using fluorescent labelled lectins to elucidate their glycan-binding properties. Histochemical analyses showed the diversity of glycan patterns in the annelid. Mannose at the non-reducing terminus of glycans was found specifically at the periphery of the intestinal wall in addition to the whole tissue. The response of glycans with non-reducing terminal N-acetylglucosamine detected by Wheat germ agglutinin was weak on the tissue section. Contrastively, glycans with fucose detected by Ulex europaeus agglutinin-1 were present in limited parts of the notopodia. At the same time, Galβ1-3GalNAc/GlcNAc and β-galactoside appeared at the circular muscles surrounding the body and were detected by Agaricus bisporus agglutinin and Erythrina cristagalli agglutinin, respectively in the same region; NeuAcα2-6Gal/GalNAc glycan was detected by Sambucus sieboldiana agglutinin. However, NeuAcα2-3Gal/GalNAc was not present in the tissue of lugworm by Maackia amurensis agglutinin. These results indicated that β-galactoside glycans were expressed in the circular muscles of the polychaeta, with a partial presence of sialic acid. The glycoproteins in the annelid were isolated from the crude extract by lectin-conjugated agarose gels. The presence of glycoconjugates expressed in P. nuntia with characteristic glycan structures is a novel finding.