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BioMed Central, Biological Procedures Online, 1(15), 2013

DOI: 10.1186/1480-9222-15-12

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Exploration of two methods for quantitative Mitomycin C measurement in tumor tissue in vitro and in vivo

Journal article published in 2013 by Lee MacKenzie Fischer, Juan Luis Vásquez, Julie Gehl ORCID, Gregers G. Hermann, Niels B. Larsen ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Two methods of quantifying Mitomycin C in tumor tissue are explored. A method of ultravioletvisible absorption microscopy is developed and applied to measure the concentration of Mitomycin C in preserved mouse tumor tissue, as well as in gelatin samples. Concentrations as low as 60 µM can be resolved using this technique in samples that do not strongly scatter light. A novel method for monitoring the Mitomycin C concentrations inside a tumor is developed, based on microdialysis and ultraviolet-visible spectroscopy. A pump is used to perfuse a microdialysis probe with Ringer's solution, which is fed to a flow cell to determine intratumor concentrations in real time to within a few µM. The success and limitations of these techniques are identified, and suggestions are made as to further development. To the authors' knowledge these are the first attempts made to quantify Mitomycin C concentrations in tumor tissue.