Published in

Principles of Cloning, p. 379-389

DOI: 10.1016/b978-0-12-386541-0.00030-8

Nature Research, Nature Methods, 1(4), p. 23-25, 2006

DOI: 10.1038/nmeth973

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Targeted Chromosome Elimination from ES-Somatic Hybrid Cells

Journal article published in 2006 by Hiroyuki Matsumura, Kentaro Yasuchika, Masako Tada, Kunio Hirano, Tomomi Otsuji, Liang Tso Sun, Norio Nakatsuji, Azim Surani ORCID, Takashi Tada
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

To engineer a stem cell genome, we developed a technology for targeted elimination of chromosomes from mouse embryonic stem (ES)-somatic hybrid cells. Here we demonstrate the use of a universal chromosome elimination cassette (CEC) for elimination of a single embryonic stem cell (ESC)-derived chromosome 11 or 12, and also both copies of chromosome 6, which harbor pluripotency-associated genes including Nanog. We attribute hybrid-cell pluripotency to the expression of Nanog from the reprogrammed somatic-cell nuclei.