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Public Library of Science, PLoS ONE, 9(7), p. e46171, 2012

DOI: 10.1371/journal.pone.0046171

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R26R-GR: A Cre-Activable Dual Fluorescent Protein Reporter Mouse

Journal article published in 2012 by Ming-Shian Tsai, Tsung-Lin Yang, Amy Tsu Ku, Ke-Han Huang, Cheng-Yen Huang, You-Tzung Chen ORCID, Fu-Ju Chou, Hsiang-Hsuan Fan, Jin-Bon Hong, Shuo-Ting Yen, Wei-Le Wang, Chang-Ching Lin, Yu-Chen Hsu, Kang-Yi Su ORCID, I.-Chang Su and other authors.
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Green fluorescent protein (GFP) and its derivatives are the most widely used molecular reporters for live cell imagining. The development of organelle-specific fusion fluorescent proteins improves the labeling resolution to a higher level. Here we generate a R26 dual fluorescent protein reporter mouse, activated by Cre-mediated DNA recombination, labeling target cells with a chromatin-specific enhanced green fluorescence protein (EGFP) and a plasma membrane-anchored monomeric cherry fluorescent protein (mCherry). This dual labeling allows the visualization of mitotic events, cell shapes and intracellular vesicle behaviors. We expect this reporter mouse to have a wide application in developmental biology studies, transplantation experiments as well as cancer/stem cell lineage tracing.