Detached 3-week-old leaves of Agrostis palustris, Lolium perenne, Poa annua, and Poa pratensis were inoculated with conidial suspensions of two isolates of Colletotrichum graminicola obtained from A. palustris. Inoculated leaves were incubated at 23 °C under high relative humidity (>95%). The infection process was investigated by light microscopy from 2 to 168 h after inoculation (AI). Spore germination was observed within 2 h AI, appressoria within 6 h AI, and penetration pores within 8 h AI on all four hosts. Infection hyphae were observed inside epidermal cells within 24 h AI on all four hosts, but significantly greater infection was observed in A. palustris and P. annua than in L. perenne or P. pratensis at both 96 and 120 h AI. Acervuli appeared on leaves of A. palustris at 72 h AI and on L. perenne at 96 h AI but were not found on either P. annua or P. pratensis during the first 168 h AI. The infection process was similar to that reported for C. graminicola from other hosts; however, disease development of the two isolates of C. graminicola from A. palustris was faster or fungal growth more extensive on detached leaf tissue of A. palustris than on other turfgrass species tested.Key words: annual bluegrass, anthracnose, creeping bentgrass.