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Florida Entomological Society, Florida Entomologist, 4(97), p. 1794-1804, 2014

DOI: 10.1653/024.097.0455

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Metarhizium anisopliaeandBeauveria bassiana(Hypocreales: Clavicipitaceae) are Compatible withCotesia flavipes(Hymenoptera: Braconidae)

This paper was not found in any repository, but could be made available legally by the author.
This paper was not found in any repository, but could be made available legally by the author.

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Data provided by SHERPA/RoMEO

Abstract

The aim of this study was to evaluate the effects of commercially available bioinsecticides based on Metarhizium anisopliae (Metschnikoff) Sorokin and Beauveria bassiana (Balsamo) Vuillemin, i.e., Biometha WP Plus® (M. anisopliae), Biovéria G® (B bassiana), Boverril WP® (B. bassiana), Metarril WP® (M. anisopliae), and Metiê WP® (M. anisopliae) on the pupae and adults of Cotesia flavipes (Cameron) (Hymenoptera: Braconidae) at concentrations of 1 × 109, 5 × 109, and 10 × 109 conidia mL−1. This braconid is released to control the sugarcane borer, Diatraea saccharalis. In the completely randomized first experiment with each commercial product, 10 C. flavipes female adults were held individually in disposable cups, which contained a 9-cm2 sugarcane leaf that had been treated with the one of the entomopathogenic fungal products. The mortality of C. flavipes females was assessed at 24, 48, 72, 96, and 120 h after treatment. In the second experiment, the same treatments were applied to C. flavipes pupae, because the latter can be exposed when the fungal products are applied to sugarcane to control various pests. In the second experiment we assessed the emergence of adults from treated pupae, the capacity of these adults to parasitize Diatraea saccharalis caterpillars, numbers of progeny of these C. flavipes, longevity of C. flavipes males and females, total adults emerged, and the percent emergence and longevity of males and females of the F1 generation. The mortality levels of C. flavipes pupae and adults were not affected by the 2 Entomopathogenic fungi. Therefore the use of Beauveria bassiana and M. anisopliae to protect sugarcane is compatible with the use of C. flavipes to suppress D. saccharalis. O objetivo deste estudo foi avaliar o efeito de bioinseticidas comercialmente disponíveis com base em Metarhizium anisopliae (Metschnikoff) Sorokin e Beauveria bassiana (Balsamo) Vuillemin (Hypocreales: Clavicipitaceae) Biometha WP Plus® (M. anisopliae), Biovéria G® (B bassiana), Boverril WP® (B. bassiana), Metarril WP® (M. anisopliae), e Metiê WP® (M. anisopliae) em pupas e adultos de Cotesia flavipes (Cameron) (Hymenoptera: Braconidae) nas concentrações de 1 × 109, 5 × 109 e 10 × 109 con mL−1. No primeiro experimento, para cada produto comercial, dez fêmeas de C. flavipes foram individualizadas em copos descartáveis e sua superfície de contato dentro (9 cm2 folha de cana de açúcar) foram tratados com o produto. O delineamento experimental foi inteiramente casualizado (DIC), com 16 tratamentos e cinco repetições, cada repetição, incluindo 10 fêmeas. A mortalidade das fêmeas de C. flavipes foi avaliada depois de 24, 48, 72, 96, e 120 horas após o tratamento com os produtos. No segundo experimento DIC, os bioinseticidas e as concentrações foram mantidas as mesmas que no primeiro experimento, com 16 tratamentos e 10 repetições, cada repetição usando um número de pupas com um potencial de emergência de 50 C. flavipes adultos. A emergência, o parasitismo, a progênie, a longevidade de machos e fêmeas, o total de adultos emergidos, e a longevidade de machos e fêmeas da geração filial (F1) deste parasitoide foram avaliados. A mortalidade de pupas e adultos de C. flavipes não foi afetada pelos fungos. B. bassiana e M. anisopliae são compatíveis com o parasitoide C. flavipes, permitindo assim a associação dessas duas formas de controle. View this article in BioOne