Dissemin is shutting down on January 1st, 2025

Published in

Optica, Optics Letters, 13(37), p. 2490, 2012

DOI: 10.1364/ol.37.002490

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Label-free in vivo flow cytometry in zebrafish using two-photon autofluorescence imaging

Journal article published in 2012 by Yan Zeng, Jin Xu, Dong Li, Li Li, Zilong Wen, Jianan Y. Qu ORCID
This paper was not found in any repository, but could be made available legally by the author.
This paper was not found in any repository, but could be made available legally by the author.

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Data provided by SHERPA/RoMEO

Abstract

We demonstrate a label-free in vivo flow cytometry in zebrafish blood vessels based on two-photon excited autofluorescence imaging. The major discovery in this work is the strong autofluorescence emission from the plasma in zebrafish blood. The plasma autofluorescence provides excellent contrast for visualizing blood vessels and counting blood cells. In addition, the cellular nicotinamide adenine dinucleotide autofluorescence enables in vivo imaging and counting of white blood cells (neutrophils).