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American Society for Microbiology, Journal of Clinical Microbiology, 6(47), p. 1950-1952, 2009

DOI: 10.1128/jcm.00626-09

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Evaluation of Low-Colony-Number Counts of Mycobacterium tuberculosis on Solid Media as a Microbiological Marker of Cross-Contamination

Journal article published in 2009 by F. K. C. Ribeiro, E. M. Lemos, D. J. Hadad, S. C. Leão ORCID, C. Viana-Niero, R. Dietze ORCID, J. L. Johnson, K. D. Eisenach, M. Palaci
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT Low-colony-number counts on solid media are considered characteristic of cross-contamination, although they are normally observed in true-positive cultures from some groups of patients. The aim of this study was to evaluate low-yield growth cultures as a microbiological marker for cross-contamination. We evaluated 106 cultures with <15 colonies from 94 patients, and the proportions of false-positive cultures were 0.9% per sample and 1.1% per patient, which indicates that low-yield growth is not a reliable marker of cross-contamination.