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Nature Research, Nature Methods, 12(12), p. 1135-1138, 2015

DOI: 10.1038/nmeth.3617

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A strategy for dissecting the architectures of native macromolecular assemblies

Journal article published in 2015 by Yi Shi, Riccardo Pellarin, Peter C. Fridy ORCID, Javier Fernandez-Martinez ORCID, Mary K. Thompson, Yinyin Li, Qing Jun Wang, Andrej Sali, Michael P. Rout, Brian T. Chait
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

It remains particularly problematic to define the structures of native macromolecular assemblies, which are often of low abundance. Here we present a strategy for isolating complexes at endogenous levels from GFP-tagged transgenic cell lines. Using cross-linking mass spectrometry, we extracted distance restraints that allowed us to model the complexes' molecular architectures.