Pseudomonas chlororaphisPCL1606 synthesizes the antifungal antibiotic 2-hexyl, 5-propyl resorcinol (HPR), which is crucial for the biocontrol of fungal soil-borne pathogens. The genetic basis for HPR production lies in thedargenes, which are directly involved in the biosynthesis of HPR. In the present study, we elucidated the genetic features of thedargenes. Reverse transcription PCR experiments revealed an independent organization of thedargenes, except fordarBC,which was transcribed as a polycistronic mRNA.In silicoanalysis of each gene revealed putative promoters and terminator sequences, validating the proposed gene arrangement. Moreover, experiments utilizing 5′ rapid amplification of cDNA ends were used to determine the transcriptional initiation sites for thedarA,darBC,darSanddarRgene promoters, and subsequently to confirm the functionality of these regions. The results of quantitative real-time PCR experiments indicated that biosyntheticdargenes were not only modulated through the global regulatorgacS, but also throughdarSanddarR. The interplay betweendarSanddarRrevealed transcriptional cross-inhibition. However, these results also showed that other regulatory parameters play a role in HPR production, such as the environmental conditions and additional regulatory genes.