In this study, we evaluated the antibacterial activity of parsley and basilic essential oils tested against Vibrio strains and their abilities to inhibit and eradicate the mature biofilm using the XTT assay. Petroselinum crispum essential oil was characterized by 1,3,8-p-menthatriene (24,2%), ß-phellandrene (22,8%), apiol (13,2%), myristicin (12,6%) and terpinolene (10,3%) as a major constituents. While, in the basilic oil, linalool (42,1%), (E)-methylcinnamate (16,9%) and 1-8 cineole (7.6%) were the main ones. These two essential oils exhibit high anti-Vibrio spp. activity with varying magnitudes. All microorganisms were strongly affected indicating an appreciable antimicrobial potential of basilic with a diameter of inhibition zones growth ranging from 8.67 to 23.33 mm and MIC and MBC values ranging from (0.023-0.047 mg/ml) and (>3->24 mg/ml), respectively. The two essential oils can inhibit and eradicate the mature biofilm formed on polystyrene surface even at low concentrations, with high magnitude for O. basilicum essential oil. This study gives a better insight into the anti-Vibrio activity of parsley and basilc oils and the possibility of their use to prevent and eradicate contamination of sea products by these strains.