The simultaneous presence of fungi and particles in horse stable environment can create a singular exposure condition because particles have been reported has a good carrier for microorganisms and their metabolites. This study intends to characterize this setting and to recognize fungi and particles occupational exposure. Air samples of 50L were collected through an impaction method and surfaces samples were also collected by swabbing the surfaces of the same indoor sites. Particles measurements were performed in different tasks using portable direct-reading hand-held equipment. Round pen was the sampling site with the highest fungal load in air and the closed box during litter changing and the feed warehouse has the highest fungal load in surfaces samples. The higher median value for PM10 and PM5 was obtained in the closed box during the horse brushing task (p<0.000). The results obtained in this study highlight the need of further and more detailed research.