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Elsevier, BBA - Bioenergetics, 3(1231), p. 215-219, 1995
DOI: 10.1016/0005-2728(95)00098-4
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Cytochrome b-558 α-subunit cloning and expression in rat aortic smooth muscle cells
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Abstract
Recent studies have shown that the NADPH oxidase participates in the generation of superoxide anion in non-phagocytic cells. Here we report the isolation and nucleotide sequence of a cDNA for the cytochrome b-558 alpha-subunit of the NADPH oxidase in rat vascular smooth muscle cells (VSMCs). The coding region of the cDNA was 93% homologous to mouse and 81% to human in nucleotide sequence and 96% homologous to mouse and 89% to human in the deduced amino acid sequence. Our results provide a tool with which to explore the mechanism of superoxide anion generation in rat VSMCs and other non-phagocytic cells.