The distribution of diatoms with respect to salinity and ionic gradients was studied in lakes of the northern Great Plains of North America. The lakes range from freshwater to hypersaline (0.65–270 g∙L⁻¹) and include a variety of brine types, although the majority are dominated by sulfate salts. Canonical correspondence analysis of diatoms in the surface sediments of 66 lakes and associated water chemistry data indicates that diatom distributions are highly correlated with salinity. The ordination also suggests that brine type forms a significant environmental gradient and separates taxa characteristic of bicarbonate/carbonate lakes from those of sulfate-dominated systems. The salinityoptima and tolerances of diatom species are calculated by weighted averaging regression, and these data provide a tool for the reconstruction of past salinity and the inferance of climatic change in arid and semiarid regions.