Published in

Oxford University Press, Nucleic Acids Research, 12(3), p. 3397-3408, 1976

DOI: 10.1093/nar/3.12.3397

Links

Tools

Export citation

Search in Google Scholar

Nucleoside 3′phosphotriesters as key intermediates for the oligoribonucleotide synthesis. III. An improved preparation of nucleoside 3′-phosphotriesters, their 1H NMR characterization and new conditions for removal of 2-cyanoethyl group

Journal article published in 1976 by R. W. Adamiak ORCID, M. Z. Barciszewska, E. Biala, K. Grzéskowiak, R. Kierzek, A. Kraszewski, W. T. Markiewicz, M. Wiewiórowski
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

Full text: Download

Green circle
Preprint: archiving allowed
Upload
Green circle
Postprint: archiving allowed
Upload
Green circle
Published version: archiving allowed
Upload
Policy details
Data provided by SHERPA/RoMEO

Abstract

An improved procedure for the transformation of 5'-O-monomethoxytrityl-2'-O-acetyl-3'-phosphates of uridine la, inosine ib and 6-N-benzoyladenosine lc into corresponding 3'/2,2,2-trichloroethyl, 2-cyanoethyl/-phosphates iiaic is reported. H NMR characterization of nucleoside 3'-phosphotriesters is presented. New conditions i.e. anhydrous triethylamine-pyridine treatment have been found for the selective removal of 2-cyanoethyl group from nucleoside 3'-phosphotriesters in the presence of neighbouring 2'-O-acetyl one.