Dissemin is shutting down on January 1st, 2025

Published in

Elsevier, Preventive Veterinary Medicine, 3-4(121), p. 391-394, 2015

DOI: 10.1016/j.prevetmed.2015.08.016

Links

Tools

Export citation

Search in Google Scholar

First genetic identification of Cryptosporidium parvum subtype IIaA14G2R1in beef cattle in Brazil

Journal article published in 2015 by Rafael Pereira Heckler, Ricardo Antônio Amaral de Lemos, Fernando de Almeida Borges, Dyego Gonçalves Lino Borges, Flávia Barbieri Bacha, Marcel Kenzo Vilalba Onizuka, Letícia e. Silva Teruya, Juliana Paniago Lordelo Neves, Cássia Rejane Brito Leal, Ricardo Antônio Amaral de Lemos, Marcelo Vasconcelos Meireles ORCID, Fernando de Almeida Borges
This paper is available in a repository.
This paper is available in a repository.

Full text: Download

Green circle
Preprint: archiving allowed
Upload
Orange circle
Postprint: archiving restricted
Upload
Red circle
Published version: archiving forbidden
Policy details
Data provided by SHERPA/RoMEO

Abstract

The presence of Cryptosporidium spp. in a cattle herd registered with an outbreak of diarrhea was investigated and the the molecular subtyping of Cryptosporidium parvum was characterized. Fecal samples from 85 Nellore beef cattle (Bos indicus) were collected and examined with Ziehl-Neelsen modified staining method. Fifty-four cattle (63.52%) had Cryptosporidium spp. oocysts in their feces. Fragments of genes encoding the 18S ribosomal RNA subunit and a 60-kDa glycoprotein (gp60) were amplified by nested PCR accomplished in the 11 most heavily parasitized samples, and the amplicons were sequenced. Eight of the 11 analyzed samples were positive for 18S rRNA sequences and identified monospecific infections with C. parvum. Seven samples were positive for gp60 and identified subtypes IIaA15G2R1 (6/11) and IIaA14G2R1 (1/11). This report is the first for C. parvum subtype IIaA14G2R1 in beef cattle in Brazil.