Published in
Humana Press, Methods in Molecular Biology, p. 189-204, 2010
DOI: 10.1007/978-1-59745-248-9_13
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Inhibition of Gene Function in Mammalian Cells Using Short-Hairpin RNA (shRNA)
Journal article published in 2010 by
Jørn Remi Henriksen,
Jochen Buechner 
,
Cecilie Løkke,
Trond Flægstad,
Christer Einvik
,
Cecilie Løkke,
Trond Flægstad,
Christer Einvik
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Abstract
RNAi is now the preferred method for silencing gene expression in a variety of systems. In this chapter we describe the procedure for applying short-hairpin RNA (shRNA) to study gene function. Detailed descriptions of target site selection, shRNA construction, shRNA transfection and target knockdown validation are included.