In order to mimic the function of catecholase activity, in-situ copper (II) complexes of three ligands containing different numbers of benzyl groups: (E)-4-methoxy-N-(4-methoxybenzylidene)aniline L 1 , (E)-N-(4-methoxybenzylidene) naphthalene-1-amine L 2 , (E)-N-(4-methoxybenzylidene)aniline L 3 , were reported and examined, in combination with different cupric salts, for their catecholase activities at ambient conditions. The highest rate activity was given by the combination of two equivalents of L 3 and one equivalent of Cu(CH 3 COO) 2 in methanol. The Michaelis-Menten model was applied to obtain the kinetic parameters of the best catalyst.