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Oxford University Press, Nucleic Acids Research, 7(39), p. 2671-2677, 2010

DOI: 10.1093/nar/gkq1190

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Cyclin E is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells

Journal article published in 2010 by Jennifer Munkley, Nikki A. Copeland, Victoria Moignard, John R. P. Knight, Erin Greaves, Simon A. Ramsbottom, Mary E. Pownall, Jennifer Southgate ORCID, Justin F.-X. Ainscough, Dawn Coverley
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Cyclin E supports pre-replication complex (pre-RC) assembly, while cyclin A-associated kinase activates DNA synthesis. We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells. In murine embryonic stem cells, Xenopus embryos and human urothelial cells, cyclin E is recruited to the nuclear matrix as cells differentiate and this can be manipulated in vitro. This suggests that pre-RC assembly becomes spatially restricted as template usage is defined. Furthermore, failure to become restricted may contribute to the plasticity of cancer cells.