In recent years, there has been a steady increase in the number of bioretention systems installed worldwide. However, there has only been limited research on the long-term effectiveness of these sustainable urban drainage system devices. This paper presents the results of a series of controlled field experiments investigating the pollutant removal efficiency of three bio-filtration system that have been in service for over five years in the Sunshine Coast in Australia. The results of this study suggest that the long-term pollution removal performance of these systems may not be as effective as previously thought and further research is needed.